广西医学2013，Vol.35Issue(1)：1-4,4.DOI:10.11675/j.issn.0253-4304.2013.01.01
二阶段化学诱发小鼠肝癌模型C-MYC基因动态变化的研究
The Dynamic Change of C-MYC Gene in Two-Stage Chemical Induced Liver Cancer Model in Mice
摘要
Abstract
Objective To investigate the dynamic change of C-MYC oncogene in the process of chemical induced liver cancer in mice. Methods Sixty healthy C57Biy6J male mice were induced to establish the hepatocellular carcinoma ( HCC ) model by combination of diethylnitrosamines( DEN ), carbon tetrachloride( CC14 ) and ethanol for twenty weeks( experimental group ),and another sixty C57BL/6J mice were used as normal controls. The mice were randomly put to death every two weeks and observed the pathology of their liver tissues. Real-time fluorescent quantitative PCR was used to detect the change of expression of C-MYC gene in experimental group and control group. Results After DEN/CC14/ethanol induced for 20 weeks,HCC model of mice were successful induced. The result of real-time fluorescent quantitative PCR suggested that no significant difference was found in C-MYC mRNA between experimental group and control group of the fourth week and the sixth week( P >0. 05 ). C-MYC expression quantity was gradually increased from the eighth week, and the increasing was most significant during the eighteenth week and the twentieth week, which was higher in the experimental group than the controls( P <0. 05 ). Conclusion In the process of induced HCC model in mice, C-MYC gene expression quantity is gradually increased with induced cancer time prolongs, C-MYC gene shows abnormal high expression in HCC stage and may promote the occurrence and development of HCC.关键词
肝肿瘤/化学诱癌/C-MYC基因/小鼠/实时荧光定量PCRKey words
Hepatocellular carcinoma/Chemical induced cancer/C-MYC gene/Mice/Real-time fluorescent quantitative PCR分类
医药卫生引用本文复制引用
赵文洁,杨帆,谢裕安..二阶段化学诱发小鼠肝癌模型C-MYC基因动态变化的研究[J].广西医学,2013,35(1):1-4,4.基金项目
国家自然科学基金(81060203) （81060203）
