广西植物2012，Vol.32Issue(6)：767-773,7.DOI:10.3969/j.issn.1000-3142.2012.06.011
15N同位素稀释法评估甘蔗的生物固氮量
Using 15N isotopic dilution method to quantify the biological nitrogen fixation in sugarcane
摘要
Abstract
The quantification of biological nitrogen fixation in sugarcane was investigated with 15N isotope dilution technique in greenhouse bucket culture test,using cassava as reference plant. The experimental results showed that Ndfa accounted for 11. 3514%,and the nitrogen fixation rate was 0. 9269 g/bucket for whole growth duration of sugarcane. Ndfa and the nitrogen fixation amount in different organs of the plant showed leaf>stalk>root. Ndfa in leaf accounted for 13. 2668%,a little higher than that in the whole plant,but the difference was not significant. The percentage of nitrogen from air,fertilizer and soil occupying the total nitrogen of the plant was 11. 3514% ,7. 6857% and 80. 9629%, respectively. The nitrogen use efficiency of sugarcane was 58. 7583%. It was concluded that all organs of sugarcane could fix nitrogen through biological nitrogen fixation,and leaves could be used to estimate the amount of biological nitrogen fixation in place of whole plant of sugarcane.关键词
甘蔗/木薯/15N同位素稀释法/固氮百分率/定量/生物固氮Key words
sugarcane/cassava/15N isotope dilution technique/nitrogen-fixing percentage/quantification/biological nitrogen fixation分类
农业科技引用本文复制引用
周晓舟,李杨瑞,杨丽涛..15N同位素稀释法评估甘蔗的生物固氮量[J].广西植物,2012,32(6):767-773,7.基金项目
国家自然科学基金(30660085,31171504,31101122) （30660085,31171504,31101122）
国家科技支撑计划项目(2007BAD30B00) （2007BAD30B00）
国家国际合作项目(2008DFA30600.2009DFA30820) （2008DFA30600.2009DFA30820）
广西自然科学基金(2011GXNSFF018002) （2011GXNSFF018002）
广西科学研究与技术开发计划项目(桂科产1123008-1A,桂科能0815011) （桂科产1123008-1A,桂科能0815011）
