广西植物2012，Vol.32Issue(6)：800-805,6.DOI:10.3969/j.issn.1000-3142.2012.06.016
转拟南芥AtNPR1基因增强水稻对水稻白叶枯病和稻瘟病的抗性
Over-expression of Arabidopsis thaliana AtNPR1 gene in rice enhances the rice resistance to bacterial blight and blast diseases
摘要
Abstract
The AtNPRl gene is a key regulator of systemic acquired resistance(SAR) in Arabidopsis thaliana. Over-expression of AtNPRl in A. thaliana enhanced resistance to both bacterial and fungal diseases. To investigate the effect of AtNPRl over-expression in rice on its disease resistance, the gene was transformed as a restorer in Gui99 of Guangxi. PCR and DNA dot blot hybridization analyses revealed that 79 transgenic plants were obtained. Northern hybridization and RT-PCR analyses indicated that AtNPRl gene was over-expressed in rice. The transgenic plants were challenged with Xanthomonas oryzae pv. oryzae(Xoo) and Magnaporthe grisea, the rice bacterial blight and fungal blast pathogens,respectively. They showed significantly enhanced resistance to Xoo and M. grisea.关键词
AtNPR1基因/水稻/桂99/转化/抗病性/水稻白叶枯病菌/稻瘟病菌Key words
AtNPRl gene/rice/Gui99/transformation/disease resistance/X. oryzae pv. oryzae/M. grisea分类
生物科学引用本文复制引用
段承杰,罗荡平,罗雪梅,冯家勋..转拟南芥AtNPR1基因增强水稻对水稻白叶枯病和稻瘟病的抗性[J].广西植物,2012,32(6):800-805,6.基金项目
广西自然科学基金(桂科自0007006) （桂科自0007006）
广西科技攻关项目(桂科攻0228019-4) （桂科攻0228019-4）
