华北农学报2012，Vol.27Issue(6)：20-23,4.
大白菜开花相关基因CO的BAC克隆筛选及分析
Screening and Analysis of BAC Clones Containing Flowering Time Gene CO in Brassica campestris L.ssp.pekinensis Olsson
摘要
Abstract
The specific primers designed according to the published Chinese cabbage CO gene ( Bra008669) were used to screen the Chinese cabbage BAC library through three steps of PCR, and 3 clones containing CO gene were obtained. The 3 obtained clones were amplificated and the sequence compared with Chinese cabbage CO gene (Bra008669) , the result showed that the amplificated fragments had 99. 73% of homology with the CO gene (Bra008669) and included N-terminal and C-terminal conservated domains.关键词
大白菜/开花相关基因/CO基因/BAC克隆Key words
Chinese cabbage/Flowering time gene/ CO gene/BAC clone分类
生物科学引用本文复制引用
马爱茹,冯大领,刘立平,轩淑欣,王彦华..大白菜开花相关基因CO的BAC克隆筛选及分析[J].华北农学报,2012,27(6):20-23,4.基金项目
国家自然科学基金(31171964) （31171964）
河北省自然科学基金(C2010000677 （）
C2010000738) （）
高等学校博士学科点专项科研基金(20101302110001) （20101302110001）
