华北农学报2012，Vol.27Issue(6)：24-29,6.
大豆转录因子基因GmMYBJ7的表达及功能分析
Expressing and Functional Analysis of GmMYBJ7 from Soybean
摘要
Abstract
Plant MYB transcription factors are characterized by the presence of a structurally conserved MYB domain, which involved in the regulation of many aspects of plant growth, development, metabolism and stress responses. A new MYB gene, designated GmMYBJ7 ( GenBank accession No. DQ902864) .was isolated from soybean ( Glycine max) . The result of subcellular localization indicated that GmMYBJ7 proteins were mostly distributed in the nucleus. Semi-quantitative RT-PCR analysis indicated that the expression of GmMYBJ7 would be decreased under the abiotic stresse treatment such as UV-B radiation, drought, and high-salt. GmMYBJ7 were transformed into tobacco NC89 with Agrobacterium LBA4404 containing the plant expression vectors pCAMBIA2301-GmMYBJ7. The results showed that the average total flavonoids content decreased significantly in the positive tobacco transformants, which suggested that GmMYBJ7 was probably invoved in the basic physiological metabolism by regulating the biosynthesis of flavonoid in plants.关键词
大豆/MYB转录因子/基因表达调控Key words
Soybean/MYB transcription factors/Gene expression and regulation分类
生物科学引用本文复制引用
杨文杰,吴燕民,唐益雄..大豆转录因子基因GmMYBJ7的表达及功能分析[J].华北农学报,2012,27(6):24-29,6.基金项目
中央级公益性科研院所科研业务费专项资金(基本2008-17) （基本2008-17）
