华北农学报2012，Vol.27Issue(6)：34-37,4.
无花果ACC合成酶基因克隆及序列分析
Cloning and Sequence Analysis of a DNA Encoding ACC Synthase Gene from Ficus carica
张帅 1张明 1寇天舒 1马俊莲 1唐霞 1张子德1
作者信息
- 1. 河北农业大学食品科技学院,河北保定071001
- 折叠
摘要
Abstract
Specific primers were designed based on the the reported ACC synthase( ACS) gene sequences of Ficus carica in the GenBank. Using genome DNA extracted from Ficus carica fruits as template the specific PCR product of ACS was obtained,then the product was ligased to pGM-T easy vector with PCR,restriction enzyme digestion and sequencing. The sequencing data showed that the PCR product was 590 bp encoding 196 predicted amino acid residues. Comparison with nucleotide sequence and amino acid sequence homology of ACC oxidase gene ( DQ269492)landed were 99% and 98% .which showed the successful cloning of the ACSI synthase gene.
关键词
无花果/ACC合成酶/基因克隆/序列分析Key words
Ficus carica/ ACC synthase/ Gene clone/ Sequence analysis分类
生物科学引用本文复制引用
张帅,张明,寇天舒,马俊莲,唐霞,张子德..无花果ACC合成酶基因克隆及序列分析[J].华北农学报,2012,27(6):34-37,4.
