华北农学报2012，Vol.27Issue(6)：62-66,5.
辣椒细胞质雄性不育系和保持系线粒体DNA差异的SRAP分析
Mitochondrial DNA Difference Analysis between CMS and Its Maintainer Line of Pepper through SRAP
摘要
Abstract
SRAP ( Sequence-related amplified polymorphism) was analyzed between mitochondrial DNA of cytoplasm male sterility line 21A and its maintainer line 21B of pepper. The analysis showed that a total of 1 440 amplified fragments and nine polymorphic fragments between 100-1 000 bp were detected by applying 128 SRAP primer combinations; the polymorphic fragment percentage was 0.63% ; seven of the polymorphic fragments was detected in male sterility line 21 A. The polymorphic fragments were isolated, cloned and sequenced, blast of the 9 clones in GenBank showed that it all have homologous with energy metabolism genes. According to the sequences, three specific primers were designed to transform the SRAP marker to more stable SCAR markers,and they only detected in the 21A line,this suggested that the newly detected fragments related to cytoplasm male sterility.关键词
辣椒/细胞质雄性不育/线粒体DNA/SRAPKey words
Pepper/CMS/Mitochondrial DNA/SRAP分类
农业科技引用本文复制引用
袁稳,吴国平,王述彬,刁卫平,刘金兵,潘宝贵,戈伟..辣椒细胞质雄性不育系和保持系线粒体DNA差异的SRAP分析[J].华北农学报,2012,27(6):62-66,5.基金项目
国家大宗蔬菜产业技术体系淮安综合试验站项目(CARS-25-G-14) （CARS-25-G-14）
江苏省自然科学基金项目(BK2010464) （BK2010464）
国家科技支撑计划(2012BAD02B02):江苏省农业科技自主创新基金项目(CX(11)1004) （2012BAD02B02）
