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PEG-6000引发对老化大豆种子膜透性及保护性酶活性的影响

王曙光 赵建奎 宁幸莲 史雨刚 孙黛珍

华北农学报2012,Vol.27Issue(6):113-117,5.
华北农学报2012,Vol.27Issue(6):113-117,5.

PEG-6000引发对老化大豆种子膜透性及保护性酶活性的影响

Effect of PEG-6000 Priming on Seed Membrane Permeability and the Activity of Protection Enzyme of Aging Soybean

王曙光 1赵建奎 2宁幸莲 3史雨刚 1孙黛珍1

作者信息

  • 1. 山西农业大学农学院,山西太谷030801
  • 2. 运城市农业机电工程学校,山西运城044000
  • 3. 万荣县农业委员会,山西万荣044200
  • 折叠

摘要

Abstract

After the seeds of 3 soybean varieties, Heizhenzhu, Jindou 19,Jinda 74 were artificially aged under the air tight condition of high temperature( (48 ± I) ℃) and high humidity( 100% relative humidity ) , then primed by 10% ,20% and 30% PEG-6000, variation of membrane permeability and the activities of protection enzymes were tested. The results showed that relative electrical conductivity of treated seeds decreased and then increased at first and subsequently decreased and rose again with extension of aging time;MDA content decreased continuously. POD activity declined at first slowly, then increased, finally declined rapidly; SOD activity rose slowly at first and then rapidly declined. After seeds aged of 3 soybean varieties were primed with different concentration PEG-6000, the membrane permeability and MDA conent reduced relatively, and the activities of protection enzymes raised. It suggested that seed resistance to abiotic stress was enhanced. In particular, after the seeds with aging slightly were primed by 20% and 30% PEG-6000,abilities of resistance to aging were significantly enhanced.

关键词

大豆/老化/PEG-6000引发/电导率/保护性酶

Key words

Soybean/ Aging/ PEG-6000 priming/ Conductivity/ Protection enzyme

分类

农业科技

引用本文复制引用

王曙光,赵建奎,宁幸莲,史雨刚,孙黛珍..PEG-6000引发对老化大豆种子膜透性及保护性酶活性的影响[J].华北农学报,2012,27(6):113-117,5.

基金项目

山西省回国留学人员择优资助项目(2008056) (2008056)

华北农学报

OA北大核心CSCDCSTPCD

1000-7091

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