华北农学报2012,Vol.27Issue(6):113-117,5.
PEG-6000引发对老化大豆种子膜透性及保护性酶活性的影响
Effect of PEG-6000 Priming on Seed Membrane Permeability and the Activity of Protection Enzyme of Aging Soybean
摘要
Abstract
After the seeds of 3 soybean varieties, Heizhenzhu, Jindou 19,Jinda 74 were artificially aged under the air tight condition of high temperature( (48 ± I) ℃) and high humidity( 100% relative humidity ) , then primed by 10% ,20% and 30% PEG-6000, variation of membrane permeability and the activities of protection enzymes were tested. The results showed that relative electrical conductivity of treated seeds decreased and then increased at first and subsequently decreased and rose again with extension of aging time;MDA content decreased continuously. POD activity declined at first slowly, then increased, finally declined rapidly; SOD activity rose slowly at first and then rapidly declined. After seeds aged of 3 soybean varieties were primed with different concentration PEG-6000, the membrane permeability and MDA conent reduced relatively, and the activities of protection enzymes raised. It suggested that seed resistance to abiotic stress was enhanced. In particular, after the seeds with aging slightly were primed by 20% and 30% PEG-6000,abilities of resistance to aging were significantly enhanced.关键词
大豆/老化/PEG-6000引发/电导率/保护性酶Key words
Soybean/ Aging/ PEG-6000 priming/ Conductivity/ Protection enzyme分类
农业科技引用本文复制引用
王曙光,赵建奎,宁幸莲,史雨刚,孙黛珍..PEG-6000引发对老化大豆种子膜透性及保护性酶活性的影响[J].华北农学报,2012,27(6):113-117,5.基金项目
山西省回国留学人员择优资助项目(2008056) (2008056)