华北农学报2012，Vol.27Issue(6)：158-162,5.
重组几丁质酶对采后葡萄贮藏过程中抗病效果的研究
Effect of the Recombinant Chitinase on the Disease Resistance in Table Grape during Postharvest Storage
摘要
Abstract
The objective of this study was to determine the mechanism and disease-resistant effect of recominant chitinase on the table grape during storage. The purified recombinant chitinase was added to the wound of Crimson seedless table grape which had already been inoculated with Botrylis cinerea. The activity of disease-resistant enzyme of the treated grape was analyzed during storage at 25 ℃. The results showed that the recombinant chitinase treatment could induce the increase of POD,PPO and PAL enzyme activity,and reduce the content of MDA and induce fruit phenolics content accumulation. Recombinant chitinase could retard onset time of grey disease symptom and lower disease index during grape storage. Morbidity index of control grape reached 100% on day 6,but recombinant chitinase treated grape only had the mordity index of 47. 8% . Overall,the recombinant chitinase could induce disease-resistance of grape to Botrytis cinerea,and inhibite postharvest decay at early storage.关键词
重组几丁质酶/采后腐烂/葡萄/诱导抗病Key words
Recombinant chitinase/ Postharvest rot/ Grape/ Induce of disease resistance分类
农业科技引用本文复制引用
阎瑞香,李宁..重组几丁质酶对采后葡萄贮藏过程中抗病效果的研究[J].华北农学报,2012,27(6):158-162,5.基金项目
国家青年科学基金项目(31101362) （31101362）
