华北农学报2012，Vol.27Issue(z1)：1-5,5.
小麦高亲和性钾转运蛋白基因HKT1启动子克隆及序列分析
Cloning and Sequence Analysis of HKT1 Promoter in Wheat
摘要
Abstract
Using specific primers based on wheat HKT1 cDNA sequence (GenBank accession ;U 16709) , single BAC clone containing HKT1 genomic sequence was identified by screening Aibai Chinese Spring BAC library. 4 192 bp HKT1 5'-flanking region was isolated by sequencing BAC plasmid. Nine transcription start sites were predicted by Neural Network Promoter Prediction(NNPP) program analysis. The functional elements were analysed by PLACE program. HKT1 promoter region contains the basic elements: TATA-box, CAAT-box, and stress-induced elements; salt-response element,cold-,dehydration-,ABA- and frozen-response elements,WUN-response elements and tissue-specific elements. Cloning of HKT1 promoter showed that it is a feasible method to isolate gene promoter by screening BAC library and sequencing BAC plasmid in wheat.关键词
小麦/高亲和性钾转运蛋白/启动子Key words
Triticum aestivum L. / High-affinity potassium uptake transporter(HKT1) / Promoter分类
生物科学引用本文复制引用
李孟军,高欣娜,傅晓艺,史占良,郭进考..小麦高亲和性钾转运蛋白基因HKT1启动子克隆及序列分析[J].华北农学报,2012,27(z1):1-5,5.基金项目
转基因生物新品种培育重大专项(2011ZX08002-005) （2011ZX08002-005）
石家庄市科技支撑计划项目(12149402A) （12149402A）
