华北农学报2012，Vol.27Issue(z1)：24-26,3.
云南岩白菜资源的DNA提取和ISSR引物筛选
DNA Extraction and ISSR Primer Screening of Resources of Bergenia purpurascens in Yunnan
摘要
Abstract
Bergenia purpurascens (Hook. f. et Thorns. ) Engl. is an endangered medicinal species mainly distributed in Yunnan, Sichuan and Tibet. There are a lot of reports about its chemistry and pharmacology but no reports on its genetic diversity and conservation. To reveal the genetic diversity of B. purpurascens in Yunnan, 18 genome DNA samples of B. purpurascens collected in Yunnan province were extracted from young leaves by modified CTAB methods and the screening of primers for ISSR analysis were conducted. The concentrations of DNA samples varied from 1 387. 5 ng/μL to 12 000 ng/μL while the ratios of A260/A280 were between 1. 61 and 1. 85. There was only one band on the electrophoretogram for each DNA sample. These results confirmed that the qualities of extracted DNA samples were good and the modified CTAB methods described in this paper could be used for extracting DNAs from plant materials with abundant polyphenol and protein. Twenty-two ISSR primers which gave good amplification results were screened out from 100 primers and this provided a solid basis for exploring genetic diversity of B. purpurascens in Yunnan by ISSR molecular marker.关键词
岩白菜/云南/DNA提取/ISSR引物筛选Key words
Bergenia purpurascens (Hook. f. et Thorns. ) Engl. / Yunnan/DNA extraction/ISSR primer Screening分类
农业科技引用本文复制引用
王仕玉,郭凤根,刘芸君,刘云芬,杨生超,张应华..云南岩白菜资源的DNA提取和ISSR引物筛选[J].华北农学报,2012,27(z1):24-26,3.基金项目
云南省社会发展科技计划(2007C211M) （2007C211M）
云南省科技厅重点产业创新工程项目(2008IF004)资助 （2008IF004）
