华北农学报2012，Vol.27Issue(z1)：61-64,4.
陆地棉胞质不育系下胚轴线粒体DNA制备
Upland Cotton Cytoplasmic Male Sterile Lines Hypocotyl Mitochondria DNA Preparation
摘要
Abstract
Using Upland Cotton sterile line 104-7 A aseptic seedling hypocotyl as experimental material, this study introduced a kind of method, specifically for cotton mitochondrial DNA extraction. Compared with the etiolated seedling leaves as materials, the method greatly reduced the chloroplast DNA pollution probability. Because Upland Cotton contains large amounts of gossypol and tannin,which made mitochondrial DNA extraction difficult,in the experimental process,we targetly joined PVP,BSA and DIECA,simplified the experimental process and improved the quality and yield of mitochondrial DNA. By ultraviolet spectrophotometry, agarose gel electrophoresis and PCR detection, it proved that high purity of mitochondrial DNA was extracted by this method. The mitochondrial DNA can fully meet the requirements for follow-up experiments. All of these provide technical support for the mitochondrial gene related research.关键词
棉花/下胚轴/线粒体DNAKey words
Cotton/Hypocotyl/Mitochondrial DNA分类
农业科技引用本文复制引用
尹国,任爱民,韩秋成,崔明晖,马维军..陆地棉胞质不育系下胚轴线粒体DNA制备[J].华北农学报,2012,27(z1):61-64,4.基金项目
国家"863"计划项目(2001AA10A102) （2001AA10A102）
河北省重大技术创新项目(11227118Z) （11227118Z）
