华北农学报2012,Vol.27Issue(z1):164-168,5.
张杂谷不同生育期同工酶特性研究
Analysis of Isozymes of Different Growing Stages of Hybrid Millet Zhang and Their Parents
摘要
Abstract
Three isozymes of esterase ( EST ) , glutamate oxaloacetate transaminase ( GOT) and glutamate dehy-drogenase(GLD) were performed by means of polyacrylamide gel electrophoresis to study the formating mechanism of heterosis of Foxtail Millet using hybrid Millet Zhang No. 3,5 ,6 and their parents respectively. The results indicated that the isozyme bands of EST, GOT and GLD were 14,7 and 1 respectively in hybrid Millet Zhang and their parents. The tested individuals and parents differed in number, Rf, activity or width of the EST and GOT bands. Esterase isozyme bands type of hybrid Millets were mainly parental complementary, zymogram pattern of both GOT and GLD in hybrid Millets were same as their parents. There is a correlation between the isozymes of patterns of EST and GOT and the formation of heterosis in hybrid Millet Zhang.关键词
张杂谷/酯酶/谷草转氨酶/谷氨酸脱氢酶/同工酶Key words
Hybrid Millet Zhang/ EST/ GOT/ GLD/ Isozyme分类
农业科技引用本文复制引用
郭秀林,张春锋,王云,刘子会,赵治海..张杂谷不同生育期同工酶特性研究[J].华北农学报,2012,27(z1):164-168,5.基金项目
国家科技支撑计划(2011BAD06B00) (2011BAD06B00)
河北省应用基础研究计划重点基础研究项目(11965517D) (11965517D)