华北农学报2012，Vol.27Issue(z1)：250-253,4.
一株产α-淀粉酶细菌的分离、鉴定及酶学性质初步研究
Screening, Identification and Characterization of an α-amylase-Producing Bacillus subtilis 2N02
摘要
Abstract
An α-amylase-producing strain was isolated from Dendrolimu intestine by plating method and screening with iodine solution. The strain 2NO2 was identified as Bacillus subtilis based on 16S ribosomal DNA sequence alignment analysis in the GenBank database. After shaking flask culture, the yield of α-amylase reached 20. 0 U/mL. The enzyme properties of the strain were examined after ammonium sulfate precipitation and dialysis for desalting. The results showed that the optimum temperature and pH of the enzyme were 50 C and 6. 5 respectively, the enzyme activity was stimulated by Mg2+ and Ca2+ , while obviously inhibited by Zn2+ and EDTA. This study provided a good experimental material for the further industrial utilization about α-amylase production.关键词
α-淀粉酶/鉴定/酶学性质Key words
a-amylase/Identification/Enzyme property分类
农业科技引用本文复制引用
孙佑赫,周开艳,熊智..一株产α-淀粉酶细菌的分离、鉴定及酶学性质初步研究[J].华北农学报,2012,27(z1):250-253,4.基金项目
国家自然科学基金(31100007) （31100007）
