华北农学报2012，Vol.27Issue(z1)：254-258,5.
云南松毛虫肠道产纤维素酶菌株的筛选鉴定及酶学性质
Screening and Identification of Cellulase-producing Strains from Intestinal Canal of Dendrolimu houi and Research on Cellulase Characterization
摘要
Abstract
Twenty-eight Cellulase-producing strains were isolated from intestinal canal of Dendrolimu houi. Basing on biochemical, physiological characterization and 16S rRNA sequences blasting, these strains were classified into Enterobacter sp. , Bacillus sp. ,Klebsiella sp. and Yokenella sp. . Strain DK4 was found as a higher-yield strain for cellulase in these strains. A phylogenetic tree was constructed with the published 16S rRNA sequences of relative bacteria species. In the phylogenetic tree,the strain DK4 was in the branch with the Bacillus cereus strain NAV-10 ( GU056811. 1). The highest cellulase activity of DK4 strain was 19. 24 U/mL when grown at 35? ,pH 7.0 for 70 h.关键词
肠道细菌/云南松毛虫/纤维素酶/鉴定/酶学性质Key words
Intestinal bacteria/Dendrolimu houi/Cellulase/Identification/Enzyme characterization分类
农业科技引用本文复制引用
孙佑赫,周开艳,熊智..云南松毛虫肠道产纤维素酶菌株的筛选鉴定及酶学性质[J].华北农学报,2012,27(z1):254-258,5.基金项目
国家"十一五"重大科技支撑计划课题基金项目(No.2008BAD95B09) （No.2008BAD95B09）
