华北农学报2012，Vol.27Issue(z1)：358-362,5.
常用化学农药对球孢白僵菌HFW-05菌株的影响及其混用对小菜蛾的联合毒力
Effects of Chemical Pesticides on Entomopathogenic Fungus Beauveria bassiana HFW-05 Strain and the Joint Toxicity of Their Mixtures against the Diamondback Moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)
摘要
Abstract
Beauveria bassiana HFW-05 strain is highly virulent against Bamisia tabaci. To broaden its insecti-cidal range and determine the joint toxicity of the mixtures of conidia spores and chemical pesticides, the bioassay of HFW-05 strain to 2nd and 3rd instar larvae of diamondback moth,Plutella xylostella(L. ) and the conidia germination and mycelia growth of HFW-05 were carried out. The results showed that HFW-05 strain was highly toxic to larvae of diamondback moth. The corrected motality of conidia spores of the concentration 5 10VmL to 2nd and 3rd in-star larvae 6 d after infection were 86. 7% and 76. 7% separately, and the toxicity to the 2nd instar larvae was markerdly higher than to 3rd instar larvae at all tested concentration. The inhibitory action of chemical agents on spores was significantly reduced with decreasing concentration. There was no significant correlation between the impact of chemicals on spores and the toxicity of fungus mixed drug to P. xylostella. At conventional dosage, chlorflua-zuron,cyhalothrin and phoxim strongly inhibited spores growth with 0,0 and 7.9% germination respectively. The virulence of 5-fold dilution of phoxim, methomyl and diflubenzuron Ⅲ mixed 1 x l06/mL spores to the P. xylostella exhibited antergic with corrected mortality 23. 4% , 16. 2% and 20. 5% respectively. Hut the other agents showed additive effect for the toxicity to P. xylostella. Chlorfluazuron and fipronil showed significant synergistic activity with corrected mortality 93.3% and 98.0% separately. This result may be used to improve biocontrol of the pest.关键词
球孢白僵菌/小菜蛾/化学农药/联合作用/增效作用Key words
Beauveria bassiana/Plutella xylostella (L. ) /Chemical pesticide/Joint toxicity/Synergistic activity分类
农业科技引用本文复制引用
曹伟平,宋健,陆晴,魏利民,冯书亮,杜立新..常用化学农药对球孢白僵菌HFW-05菌株的影响及其混用对小菜蛾的联合毒力[J].华北农学报,2012,27(z1):358-362,5.基金项目
国家"863"项目(2011AA10A203) （2011AA10A203）
国家"863"项目(2011AA10A201) （2011AA10A201）
国家"948"项目(2011-G4) （2011-G4）
河北省财政项目(F12C10034). （F12C10034）
