郑州大学学报（医学版）2012，Vol.47Issue(6)：759-761,3.DOI:10.3969/j.issn.1671-6825.2012.06.004
纳米活性炭吸附多烯紫杉醇对A549细胞增殖及凋亡的影响
Effects of docetaxel-loaded activated carbon nanoparticles on proliferation and apoptosis of A549 cells
摘要
Abstract
Aim:To study the effects of docetaxel( DOC )-loaded activated carbon nanoparticles( ACNP-DOC ) on proliferation and apoptosis of human lung cancer A549 cells. Methods: A549 cells were teated with 0. 2,1. 0,5. 0,25. 0 and 125.0 μmol/L DOC or ACNP-DOC for 12,24,48 and 72 h,respectively,then the proliferation inhibition rate was assayed by MTT. A549 cells were teated with ACNP-DOC,DOC and ACNP at dose of IC50 for 24 h,then the apoptosis rate was detected by flow cytometry. Results:DOC and ACNP-DOC in the concentration range of 0. 2 ~ 125. 0 μmol/L could inhibit A549 cell proliferation in a time- and concentration-dependent manner, but the effects of ACNP-DOC were significantly stronger( Fdrug = 12. 326 ,Ftime = 76. 247 , Fdose = 53. 028 , Finteraction = 20. 842,P < 0. 05 ). IC50 of ACNP-DOC and DOC was 0. 80 and 43. 45 μmol/L,respectively. The apoptosis rate of A549 cells treated with ACNP,DOC ,or ACNP-DOC at the dose of IC50 differed from each other( F = 30. 208,P < 0. 001 ), and that of ACNP-DOC group was higher than those of ACNP group and DOC group( P <0.001 ). Conclusion :ACNP-DOC can inhibit A549 cell proliferation,induce apoptosis,and the effects is stronger than DOC.关键词
纳米活性炭/多烯紫杉醇/A549细胞/凋亡/增殖Key words
activated carbon nanoparticle/ docetaxel/A549 cell/ apoptosis/ proliferation分类
医药卫生引用本文复制引用
蔡要欣,张韧铮,孙岚,宋东颖,刘贵富,张莉蓉,张英鸽..纳米活性炭吸附多烯紫杉醇对A549细胞增殖及凋亡的影响[J].郑州大学学报（医学版）,2012,47(6):759-761,3.基金项目
国家863计划项目 2006AA03Z333 （）
国家973计划项目 2010CD933904 （）
