郑州大学学报（医学版）2012，Vol.47Issue(6)：765-769,5.DOI:10.3969/j.issn.1671-6825.2012.06.006
阿托伐他汀对内皮细胞微粒诱导的人脐静脉内皮细胞ERK、p38MAPK、NF-κB p65蛋白及ICAM-1mRNA 表达的影响
Effects of atorvastatin on expressions of ERK,p38MAPK,NF-κB proteins and ICAM-1 mRNA in HUVECs induced by endothelial microparticles
摘要
Abstract
Aim:To observe the effects of atorvastatin on ERK,p38MAPK and nuclear factor-KB ( NF-KB ) and intercellular adhesion molecule 1( ICAM-1 ) expressions in endothelial microparticles( EMPs )-induced human umbilical vein endothelial cells( HUVECs ). Methods:The cultured HUVECs were cultured with EMPs of 105 mL-1 for 0,3 ,6,12,and 24 h. The HUVECs were cultured with 0,10-2,10-3,10-4,10-5 mL-1 EMPs for 24 h. The HUVECs were pretreated with ERK inhibitor ( PD98059 ),p38 inhibitor ( SB203580 ),NF-KB inhibitor ( PDTC ),or atorvastatin,then were treated with EMPs. The ICAM-1 mRNA level was detected by Real-time-PCR. Western blot was performed to determine the expression levels of phospho-ERK( p-ERK ),p-p38MAPK and NF-KB p65 proteins. Results:With the increase of dose and exposure time of EMPs,the expression levels of ICAM-1 mRNA, p-ERK, p-p38MAPK and NF-KB p65 protein in HUVECs obviously in-creased( P <0.001 ). After the pretreatment of the inhibitors mentioned above and atorvastatin,the ICAM-1 mRNA expression decreased( P <0.05 ). Conclusion: Atorvastatin could downregulate ICAM-1 expression in EMPs-induced HUVECs by blocking ERK/MAPK and NF-KB signaling pathway.关键词
内皮细胞微粒/脐静脉内皮细胞/丝裂素活化蛋白激酶/细胞间黏附分子-1/阿托伐他汀Key words
endothelial microparticle/ human umbilical vein endothelial cell/ mitogen-activated protein kinase/ intercellular adhesion molecule 1/ atorvastatin分类
医药卫生引用本文复制引用
陆永光,符春晖,严华,陈湘桂,黄军章,陈丽媛..阿托伐他汀对内皮细胞微粒诱导的人脐静脉内皮细胞ERK、p38MAPK、NF-κB p65蛋白及ICAM-1mRNA 表达的影响[J].郑州大学学报（医学版）,2012,47(6):765-769,5.基金项目
广西自然科学基金青年基金资助项目 2012GXNSFBA053113 （）
广西卫生厅自筹经费科研项目 Z2011103 （）
