郑州大学学报（医学版）2012，Vol.47Issue(6)：773-775,3.DOI:10.3969/j.issn.1671-6825.2012.06.008
肿瘤转移相关基因1HLA-A3限制性细胞毒性T淋巴细胞表位的预测与鉴定
Identification of HLA-A3 restricted cytotoxic T lymphocyte epitopes from metastasis associated gene 1
摘要
Abstract
Aim:To identify the HLA-A3 restricted cytotoxic T lymphocyte( CTL ) epitopes from metastasis associated gene 1( MTA1 ). Methods:RT-PCR was used to determine the expression of MTA1 mRNA in cancer cell lines,EC-1 ,EC-109 and T-47D. HLA-A3 epitopes from MTA1 protein were predicted by BIMAS, SYFPEITHI, NetCTL 1.2 and IEDB. Peptides were synthesized by standard Fmoc chemistry method. Their binding affinities towards HLA-A3 molecule were evaluated by T2A3 cells binding assay. Their ability to induce T cell response was investigated by using cytotoxicity assay in vitro. Results: The expression of MTA1 mRNA was observed in EC-1 ,EC-109 and T-47D cells. The candidate peptide P130 showed weak affinity towards HLA-A3 molecule, while P294 and P559 showed moderate affinity. The CTLs induced by all the three peptides could lyse EC-1 cells( Fcell = 176.107 ,Fratio = 48. 306, Finteraction =35.686,P <0.001 ). Conclusion:The peptides P130,P294, and P559 could induce anti-tumor inmmunity in vitro and serve as candidates towards antitumor peptide vaccines.关键词
肿瘤转移相关基因1/HLA-A3/细胞毒性T淋巴细胞Key words
metastasis associated gene 1/ HLA-A3 / cytotoxic T lymphocyte分类
生物科学引用本文复制引用
韩艳林,翟明霞,吴亚红,高艳锋,祁元明..肿瘤转移相关基因1HLA-A3限制性细胞毒性T淋巴细胞表位的预测与鉴定[J].郑州大学学报（医学版）,2012,47(6):773-775,3.基金项目
国家自然科学基金资助项目 81172893,30901362 （）
郑州大学研究生科学研究基金资助项目 11L00403 （）
