郑州大学学报（医学版）2012，Vol.47Issue(6)：791-793,3.DOI:10.3969/j.issn.1671-6825.2012.06.013
黄芪多糖对大剂量60Co γ射线损伤大鼠睾丸间质细胞的影响
Effects of astragalus polysacharin on rat leydig cells exposed to 60Co γ ray
摘要
Abstract
Aim:To investigate the protection of astragalus polysacharin( APS ) on the impairment of the leydig cells caused by higher doses of Co 7 in rats. Methods: Thirty male SD rats were randomized into three groups( n = 10 ):the normal control group,the radiation control group,the APS plus radiation group. The structural changes of the leydig cells were observed with light microscope. The serum luteinizing hormone( LH ) and testosterone( T ) levels were measured by ELISA; and the mRNA expression level of luteinizing hormone receptor( LHR ) from the leydig cells surface was measured by RT-PCR. Results:Compared with the normal control group,the leydig cells exhibited decreases in size,cytoplasm,and quantity as well as the nuclear shrinkage after radiation. The mRNA expression levels of LHR were lower( P <0. 01 );in the APS irrigation group,the pathological changes of leydig cells were lightly. The mRNA expression levels were close to the normal; the serum LH and T levels were no significant change. Conclusion: APS can reduce the impairment of the leydig cells caused by the higher doses 60Co γ ray in rats,which may be involved in its induce the high expression of LHR mRNA.关键词
黄芪多糖/睾丸间质细胞/60Coγ射线/大鼠Key words
astragalus polysacharin/leydig cell/ 60Co γ ray/rat分类
医药卫生引用本文复制引用
王瑞,张杰,张卫星,秦俊昌,王磊..黄芪多糖对大剂量60Co γ射线损伤大鼠睾丸间质细胞的影响[J].郑州大学学报（医学版）,2012,47(6):791-793,3.基金项目
河南省科技攻关基金资助项目 102102310067 （）
