中医学报2013，Vol.28Issue(2)：228-230,3.
顽痹清丸的质量标准研究
Study on Quality Standard of Wanbiqing Pill
杜天信 1罗石任 2杜志谦 2李洁 1张毅 1王秀真2
作者信息
- 1. 河南正骨研究院,河南洛阳471000
- 2. 河南中医学院,河南郑州450046
- 折叠
摘要
Abstract
Objective:To establish the quality standards of Wanbiqing Pill. Methods:Thin layer chromatography (TLC) was applied for the qualitative identification of Starjasmine Stem, Frankincense, Cortex moutan, and Herba Leonuri in Wanbiqing Pill and HPLC method was used to determine the content of baicalin in Wanbiqing Pill. Agilent C18 Column (4.6 mm ×250 mm,5 μm) was used as chroma-tographic column,and rnethanol -0.1% phosphoric acid (47:53) as the mobile phase. The flow rate was 1.0 mL·min-1 and the column temperature was 30℃. The detection wavelength was 280 nm. The number of theoretic plate of baicalin was not less than 5 000. Results:Starjasmine Stem,Frankincense,Cortex moutan,and Herba Leonuri could be qualitatively identificated by TLC,which had clear spots, good separating degree, strong specificity, and negativity without interference. The content determination of baicalin had good linear relation in the range of 0.277 6-2.776 0 μg,r=0.999 4;the average recovery rate was 99. 10% ,and RSD was 1.29% . Conclusion: This method is simple and feasible,accurate,reproducible,which can be regarded as the quality control of Wanbiqing Pill.关键词
顽痹清丸/薄层色谱法/高效液相色谱法/定性鉴别/含量测定/络石藤/乳香/牡丹皮/益母草/黄芩苷Key words
wanbiqing pill/TLC/HPLC/qualitative identification/content determination/starjasmine stem/frankincense/cortex moutan/ herba leonuri/ baicalin分类
医药卫生引用本文复制引用
杜天信,罗石任,杜志谦,李洁,张毅,王秀真..顽痹清丸的质量标准研究[J].中医学报,2013,28(2):228-230,3.
