哈尔滨商业大学学报（自然科学版）2012，Vol.28Issue(6)：644-646,3.
RP-HPLC法测定格列吡嗪缓释片的释放度
RP-HPLC determination of drug release of glipizide sustained release tablets
屠思远 1蒋海松 2朱德志 2王建明1
作者信息
- 1. 黑龙江中医药大学,哈尔滨150040
- 2. 哈尔滨科信必成医药科技发展有限公司,哈尔滨150040
- 折叠
摘要
Abstract
This paper established an RP - HPLC method for the determination of the drug release of glipizide sustained release tablets. The RP - HPLC was on a ODS C18 column (5 μm, 4. 6 mm × 250 mm) at room temperature, using the mobile phase of methanol - buffer solution(0. 1 mol/L sodium dihydrogen phosphate, adjusted to pH (6. 00 ±0. 05) with 2 mol/L sodium hydroxide) (55∶45) , at a flow rate of 1. 0 mL/min and the detection wavelength of 225 nm. Glipizide was detected by the apparatus Ⅲ of dissolution, using as the medium 250 mL of 0. 5% lauryl sodium sulfate solution, at 50 r/min. 5 mL sample of the medium in turn at 2, 6 and 10 h to determine following the above method. The calibration curve was linear in the range of 1. 019 ~ 26. 49 μg/mL. The method was accurate for the drug release determination of glipizide sustained release tablets. The analysis was out of interference of excipients.关键词
格列吡嗪缓释片/释放度/高效液相色谱法Key words
glipizide sustained release tablets/ release/ RP - HPLC分类
医药卫生引用本文复制引用
屠思远,蒋海松,朱德志,王建明..RP-HPLC法测定格列吡嗪缓释片的释放度[J].哈尔滨商业大学学报（自然科学版）,2012,28(6):644-646,3.
