河南农业科学2012，Vol.41Issue(9)：87-90,4.
河南省几种白粉菌的ITS序列分析
ITS Sequence Analysis of Several Powdery Mildew from Henan Province
摘要
Abstract
The DNA of five powdery mildews from Henan province were extracted by modified CTAB method,and then the rDNA ITS sequences were amplified to analyze the phylogenetic relationship. The result showed that three ITS sequences of Podosphaera section from Impatiens balsamina ,Vigna un-guiculata and Helianthus tuberosus were in one cluster for their small genetic distances and close genetic relationships, and the ITS sequences of Erysiphe sp. from Euonymus japonicus and Odium neolycopersici from tomato were in another cluster,which was consistent with the classification by the morphological characteristics. Therefore, the application of ITS sequences can provide important theoretical basis for pathogen classification and phylogenetic relationship analysis.关键词
白粉菌/亲缘关系/内转录间隔区Key words
powdery mildew/phylogenetic relationship/internal transcribed spacer (ITS)分类
生物科学引用本文复制引用
马原松,裴冬丽,王文静,李成伟..河南省几种白粉菌的ITS序列分析[J].河南农业科学,2012,41(9):87-90,4.基金项目
国家自然科学基金项目(30600413) （30600413）
河南省科技厅基础与前沿技术研究项目(122300410226) （122300410226）
河南省教育厅自然科学基金项目(2011A180024) （2011A180024）
