河南农业科学2012，Vol.41Issue(9)：133-137,5.
口蹄疫3D蛋白N端T细胞表位重组蛋白的表达、纯化及鉴定
Expression,Purification and Identification of 3D Protein N Terminal T Cell Epitope Recombinant Protein of Foot and Mouth Disease Virus
摘要
Abstract
In order to obtain the recombinant protein that contained N terminal T cell epitopes in 3D protein of FMDV.the gene was synthesized according to the sequences of N terminal 115 ami-no acids. The recombinant expression vector pET28a-115 was constructed by cloning the gene of 115 amino acids into the prokaryotic expression plasmid pET-28a. The BL2KDE3) was transformed with pET28a-115 and the protein corresponding to the gene of 115 amino acids was expressed after induction with IPTG. SDS-PAGE and Western-blot showed that the molecular mass of the protein was approximately 16 kD. The soluble analysis showed that the recombinant protein was almost solubly expressed. The high purity and activity protein was obtained after purified by Ni-NTA resins. The results showed that the recombinant protein that contained N terminal 115 amino acids of 3D protein was expressed solubly and efficiently in E. coli. The purified protein has goo igenicity, which lays foundation for developing the diagnosis antigen and genetic engineer- ing cine of FMDV.关键词
口蹄疫/3D聚合酶/T细胞表位重组蛋白/原核表达/疫苗佐剂Key words
foot and mouth disease/3D polymerase/T cell epitope recombinant protein/proka-ryotic expression/vaccine adjuvant分类
农业科技引用本文复制引用
卢清侠,王世红,金前跃,李清州,郝慧芳,张改平..口蹄疫3D蛋白N端T细胞表位重组蛋白的表达、纯化及鉴定[J].河南农业科学,2012,41(9):133-137,5.基金项目
河南省级重点实验室建设专项(122300413217) （122300413217）
河南省博士后项目一等资助. （）
