河南农业科学2012，Vol.41Issue(12)：47-51,5.
EMS、NaN3和60Coγ-射线处理对芝麻根尖的细胞学效应
Cytological Effects Induced by EMS,NaN3 and 60Co γ-ray on Sesame Seed
摘要
Abstract
In order to illuminate the cytological effects of physical and chemical mutagens on the root-tip cells of the M1 generation of sesame, mitotic index, rates of chromosome aberration and micronuclei of sesame seeds derived from three varieties,which were mutated by EMS,NaN3 and 60Co γ-rays,were observed by light microscope. The results showed that the low concentration of EMS (5 g/L) and NaN3(2 mmol/L) ,interestingly,both had a pair of opposite effects on the mitosis of tip cells,just depending on the varieties. As concentration (or dosages) and treatment time of the mutagens increasing,the mitotic index of root tip cells decreased. Furthermore,the EMS, NaN3 and 60Co γ-rays induced the chromosomal and nuclear aberration,such as single micronucle-us, double micronucleus, chromosome fragment, draggling chromosome, chromosome bridges, chromosome clump,and so on. The experimental result indicated that three sesame varieties had different sensitivity to different mutagens. Yuzhi 11 and ms86-l were more sensitive to the high-concentration EMS (15 g/L) than Sanhuang sesame; compared with the two other varieties, ms86-l had better sensitivity to the low-concentration NaN3 (2 mmol/L);60Co γ-rays exhibited the best effective role in mutating ms86-l, followed by Sanhuang sesame and Yuzhi 11.关键词
芝麻/诱变/有丝分裂指数/染色体畸变/核畸变Key words
sesame/mutagenesis/mitotic index/nuclear aberration/chromosomal aberration分类
农业科技引用本文复制引用
刘艳阳,梅鸿献,崔承齐,郑永战,张海洋..EMS、NaN3和60Coγ-射线处理对芝麻根尖的细胞学效应[J].河南农业科学,2012,41(12):47-51,5.基金项目
现代农业产业技术体系建设专项(CARS-15) （CARS-15）
