核农学报2013，Vol.27Issue(1)：21-27,7.
小麦60Co γ射线辐照后TaKu70和TaKu80基因应答模式及基因组多态性分析
TaKu70 AND TaKu80 GENES RESPONSE PATTERNS AND GENOMIC POLYMORPHISM ANALYSIS OF WHEAT IRRADIATED BY GAMMA RAYS
摘要
Abstract
60Co γ - rays irradiated seeds and seedling of two wheat genotypes Jing411 (J411) and Han6172 ( H6172) were used to study the relationship among radiation sensitivity, response patterns of TaKulO and TaKuSO genes and the genomic AFLP polymorphism in the M1 generation. The results showed that γ-rays irradiation significantly inhibited the seedling growth of wheat, and the radiation sensitivity of J411 was higher than that of H6172. TaKuSO gene expression was up-regulated in response to γ-rays irradiation in J411 and H6172, but no significant changes in the expression level of TaKuJO gene. The expression level of TaKuJO and TaKuSO genes in J411 were lower than in H6172. γ-rays irradiation could increase the genomic polymorphism of M1 in J411 and H6172, and the polymorphic frequencies increased in J411 was significantly higher than that in H6172. It was concluded that the expression level and response pattern of TaKu70 and TaKu80 genes were closely related with wheat radiation sensitivity and irradiated genomic polymorphism. Transformation of TaKu70 and TaKu80 genes may be a new approach to improve the radiation sensitivity, and to improve mutation breeding efficiency in wheat.关键词
小麦/TaKu70/TaKu80/辐照敏感性/60Co γ射线/AFLPKey words
wheat/TaKu70/TaKu80/irradiation sensitivity/60Co γ -rays/ AFLP引用本文复制引用
崔孟,古佳玉,郭会君,赵林姝,谢永盾,赵世荣,李军辉,宋希云,刘录祥..小麦60Co γ射线辐照后TaKu70和TaKu80基因应答模式及基因组多态性分析[J].核农学报,2013,27(1):21-27,7.基金项目
国家高技术研究发展计划(863计划)项目(2012AA101202和2012AA101105),农业部农业公益性行业科研专项(201103007),国际原子能机构项目(CRP15651和RAS5056) （863计划）
