庆大霉素单克隆抗体的研制及ELISA分析方法的建立OA北大核心CSCDCSTPCD
PREPARATION OF MONOCLONAL ANTIBODY AND DEVELOPMENT OF AN INDIRECT COMPETITIVE ELISA METHOD FOR GENTAMICIN
用与牛血清蛋白(BSA)交联的庆大霉素人工抗原(GM-BSA)免疫的BALB/C鼠脾细胞与SP2/0鼠骨髓瘤细胞融合,经筛选、克隆,得到1株能稳定分泌抗庆大霉素单抗的杂交瘤细胞株(6H8),经鉴定6H8的抗体类型及亚类均为IgG1,其轻链为κ链.制备单克隆抗体腹水,腹水的间接ELISA效价在1×10-7以上.该单克隆抗体与庆大霉素结构类似物均无交叉反应,具有高度特异性.以制备的单抗建立间接竞争ELISA方法,其线性回归方程为y=16.122ln(…查看全部>>
One hybridoma cell line (6H8) secreting monoclonal antibody (McAb) against gentamicin was produced by fusing mouse myeloma cells ( SP2/0) with spleen cells from BALB/C which immunized by the artificial gentamicin antigen conjugated with bovine serum albumin ( BSA). Isotype and subclass of the monoclonal antibody secreted from the hybridoma cell line (6H8) was classified as IgGl. The light chain of the McAb was identified to be Κ chain. The McAb obtained coul…查看全部>>
金仁耀;吴建祥
浙江工商大学食品与生物工程学院,浙江杭州310012浙江大学生物技术研究所,浙江杭州310058
庆大霉素单克隆抗体竞争ELISA
Gentamicin monoclonal antibody competitive ELISA
《核农学报》 2013 (1)
88-92,5
浙江省科技计划项目(2011C22078)
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