湖南中医药大学学报2012，Vol.32Issue(12)：5-7,3.DOI:10.3969/j.issn.1674-070X.2012.12.002.005.03
2型糖尿病肾阴虚证大鼠模型的建立及其稳定性评价
Establish the rat model of type 2 diabetic kidney yin deficiency syndrome and evaluate its stability
摘要
Abstract
Objective To establish a method to create a rat model of type 2 diabetic kidney yin deficiency syndrome and evaluate its stability. Methods Used the method of gavage high-fat milk and intraperitoneal injection of small doses of streptozotocin to induce the Type 2 diabetic rat model, then gavage the Hydrocortisone of Glucocorticoid establish the rat model of type 2 diabetic kidney yin deficiency syndrome, And then continue to feed a month with high-fat milk, detect the Blood glucose and cAMP levels of the rat model, study the model' s stability. Results Successfully established the rat model of type 2 diabetic kidney yin deficiency syndrome with high-fat milk, small doses of streptozotocin and hydrocortisone. The model group' s Blood glucose≥16.7mmol/L,the value of cAMP is 1.32±0.04 mmol/L, and the daily characterization of the rat is similar to the kidney yin deficiency syndrome of the Traditional Chinese Medicine. The model group' s Blood glucose≥ 16.7mmol/L and the value of cAMP is 1.24 + 0.02 mmoL/L in a month later. Conclusion Successfully copy out a good stability rat model of type 2 diabetic kidney yin deficiency syndrome by gavage high-fat milk and Intraperitoneal injection of small doses of streptozotocin and then gavage the Hydrocortisone.关键词
2型糖尿病/肾阴虚/大鼠/动物模型/评价Key words
Type 2 diabetic kidney yin deficiency syndrome/ Rat/ Animal model/ Evaluate分类
医药卫生引用本文复制引用
戴冰,杨赛男,肖子曾,何泽云,周芳亮,黄开颜,刘红宇,邢瑞..2型糖尿病肾阴虚证大鼠模型的建立及其稳定性评价[J].湖南中医药大学学报,2012,32(12):5-7,3.基金项目
湖南省科学技术厅科技计划一般项目(2010FJ4091) （2010FJ4091）
长沙市科技局项目(K1106040-31) （K1106040-31）
湖南省高校创新平台开放基金项目(11K046). （11K046）
