湖泊科学2013，Vol.25Issue(1)：131-137,7.
镍胁迫对菹草(Potamogeton crispus L.)活性氧及脯氨酸代谢的影响
Effect of nickel stress on reactive oxygen species and proline metabolism of Potamogeton crispus L.
摘要
Abstract
Sterile seedlings of Potamogeton crispus were cultured by tissue culture technique, and used as experimental materials. In this study, different concentrations of Ni2+ (0, 0. 05, 0. 10, 0. 15 and 0. 20 mmol/ L) on contents of hydrogen peroxide ( H2 O2 ) , superoxide anion ( O2- ) and malondialdehyde ( MDA) , antioxidant defense system and proline metabolism were investigated. The results indicated that (1) Ni + stress triggered the generation of reactive oxygen species ( ROS) as evidenced by a substantial accumulation of H2O2 and O2 - , accompanied by the increased MDA content with the increasing of Ni 2+ concentration; (2) Activities of superoxide dismutase (SOD), peroxides (POD) and catalase (CAT) , as well as contents of reduced glutathione (GSH) and non-protein thiols (NP-SH) increased at first and then decreased, while no outstanding changes were observed in ascorbate ( AsA) content, and level of phytochelatins ( PCs) increased significantly; (3 ) Analysis of enzyme activities showed that the accumulation of proline induced by Ni2+ stress is due to the increases of omithine-8-aminotransferase (OAT) at 0.05 -0. 10 mmol/L and pyrroline-5-carboxylate synthetase (P5CS) activity at 0. 15 - 0.20 mmol/ L, respectively, but there was not a statistically significant difference in ProDH activity. Our results demonstrated that Ni2 + induced the enhancement of reactive oxygen species level, aggravated lipid peroxidation and interfere with intrinsic balance of antioxidant defense system. In addition, pro-line accumulation is mediated by increased biosynthesis from ornithine at low concentrations (0. 05 and 0. 10 mmol/L) , while glu-tamate pathway, instead of the ornithine pathway, plays a vital role when exposed to higher concentrations of Ni2+ .关键词
菹草/Ni2+胁迫/活性氧/脯氨酸代谢Key words
Potamogeton crispus L. / Ni 2+ stress/ reactive oxygen species/ proline metabolism引用本文复制引用
陈霖,姜岩,汪鹏合,施国新,乔绪强,田秀丽..镍胁迫对菹草(Potamogeton crispus L.)活性氧及脯氨酸代谢的影响[J].湖泊科学,2013,25(1):131-137,7.基金项目
江苏高校优势学科建设工程项目(164320H106)资助. （164320H106）
