湖南农业大学学报（自然科学版）2013，Vol.39Issue(1)：63-68,114,7.DOI:10.3724/SP.J.1239.2013.00063
利用圆环病毒2结构蛋白建立检测其抗体的间接ELISA方法
An indirect ELISA established using the capsid protein of PCV2 for detection of antibodies to porcine circovirus 2
摘要
Abstract
To establish an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to PCV2, the cap gene without nuclear localization signal peptide sequence of PCV2 was cloned into the pET-28a(+) vector, and the recombinant Cap△141 protein was expressed in soluable form in Escherichia coli (E.coli).The optimum working conditions of the indirect ELISA were determined using Cap△41 protein as diagnostic antigen, and 394 pig serum samples from 4 pig farms were tested.The results showed the diagnostic sensitivity and specificity of the established indirect ELISA were 93.14% and 95.17%, respectively, compared with indirect immunofluorescence (IIF).And the inter-assay and intra-assay variations of the ELISA were low.Besides, the seroprofiles of 4 pig farms revealed by the testing results were in accordance with clinical results, indicating the established indirect ELISA is reliable and can be used for detection of antibodies to PCV2.关键词
圆环病毒2/结构蛋白/抗体检测/酶联免疫吸附试验Key words
porcine circovirus type 2/ structural protein/ antibody detection/ enzyme-linked immunosorbent assay分类
农业科技引用本文复制引用
葛猛,屈泰龙,罗维,李杰,周洪锐,余兴龙..利用圆环病毒2结构蛋白建立检测其抗体的间接ELISA方法[J].湖南农业大学学报（自然科学版）,2013,39(1):63-68,114,7.基金项目
国家自然科学基金项目(30972167) （30972167）
