湖南农业大学学报（自然科学版）2013，Vol.39Issue(1)：69-72,4.DOI:10.3724/SP.J.1239.2013.00069
羊毛尾线虫线粒体rrnL基因部分序列的克隆和进化分析
Cloning and sequence analysis of the partial mitochondrial rrnL gene of Trichuris ovis isolates in China
摘要
Abstract
The purpose of this study was to analyze sequence variation in the partial mitochondrial ribosomal large subunit gene (prrnL) among Trichuris ovis (T.ovis) isolates from Guangdong province, China and to study the phylogenetic relationships of T.ovis with other whipworms using prrnL sequences.The prrnL sequences were amplified from individual T.ovis samples by polymerase chain reaction (PCR) method and the obtained prrnL sequences were aligned using ClustalX 1.81.Maximum parsimony (MP) tree was constructed using the software PAUP 4.0 Beta 10.The lengths of all prrnL were 849-850 bp, and differences in prrnL sequences within T.ovis were 0-0.8%.Phylogenetic analyses showed that all T.ovis isolates were clustered in the same clade.The prrnL sequences were quite conserved within whipworm species while inter-species differences (33.4%-36.1%) were obvious.Thus prrraL sequences can be used as a useful marker for the differentiation and identification of different whipworms.关键词
羊毛尾线虫/线粒体DNA/rrnL基因/种系发育关系Key words
Trichuris ovis/ mitochondrial DNA/ ribosomal large subunit gene (rrnL)/ phylogenetic relationship分类
农业科技引用本文复制引用
张悦文,徐颖,王燕,朱兴全,刘国华..羊毛尾线虫线粒体rrnL基因部分序列的克隆和进化分析[J].湖南农业大学学报（自然科学版）,2013,39(1):69-72,4.基金项目
家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2010KFKT010、SKLVEB2011KFKT004) （SKLVEB2010KFKT010、SKLVEB2011KFKT004）
国家肉牛牦牛产业技术体系项目(CARS-38) （CARS-38）
