华西口腔医学杂志2012，Vol.30Issue(6)：641-644,649,5.DOI:10.3969/j.issn.1000-1182.2012.06.019
富血小板纤维蛋白与富血小板血浆体外释放生长因子的比较及其对脂肪干细胞增殖分化的影响
The comparison of platelet-rich fibrin and platelet-rich plasma in releasing of growth factors and their effects on the proliferation and differentiation of adipose tissue-derived stem cells in vitro
杨世茂 1王明国 2李静 2刘金盼 3林夏莲 3徐炜3
作者信息
- 1. 山东大学口腔医院 口腔颌面外科,济南250012
- 2. 济南市中心医院 口腔科,济南250013
- 3. 潍坊医学院 口腔医学院,潍坊261053
- 折叠
摘要
Abstract
Objective To compare the releasing of growth factors between platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) as well as their effects on the proliferation and differentiation of adipose tissue-derived stem cells (ADSCs) in vitro. Methods Blood was taken from central artery of rabbits, acquiring PRF was acquired through one time centrifuge and PRP through two times centrifuge. Five milliliters of fresh α-MEM was added to PRF and PRP and incubated at 37 ℃. The time points to collect exudates was in day 1, 7, 14, 21, 28 and the mass concentrations of transforming growth factor-β1 (TGF-β1) and platelet derived growth faetor-AB (PDGF-AB) were quantified in PRF and PRP. Then the exudates of PRF and PRP were used to culture ADSCs and evaluate the effects of PRF and PRP on proliferation and differentiation of ADSCs. Results 1) Growth factor release: In the PRF exudates at different time points, the mass concentration of TGF-β1 was the highest at day 14 and the highest mass concentration of PDGF-AB at day 7, the mass concentration of both TGF-β1 and PDGF-AB was the highest at the first day and then gradually declined. 2)The effect on proliferation and differentiation: PRF exudates of day 14 expressed the maximum proliferation and alkaline phosphatase (ALP) activity. PRF exudates of day 1 demonstrat the maximum proliferation and ALP activity. Conclusion Comparing to PRP, PRF releases growth factors gradually and expressed stronger and more durable effect on proliferation and differentiation of ADSCs in vitro.关键词
富血小板纤维蛋白/富血小板血浆/脂肪干细胞/成骨分化Key words
platelet-rich fibrin/ platelet-rich plasma/ adipose tissue-derived stem cell/ osteogenic differentiation分类
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杨世茂,王明国,李静,刘金盼,林夏莲,徐炜..富血小板纤维蛋白与富血小板血浆体外释放生长因子的比较及其对脂肪干细胞增殖分化的影响[J].华西口腔医学杂志,2012,30(6):641-644,649,5.
