华西口腔医学杂志2012，Vol.30Issue(6)：645-649,5.DOI:10.3969/j.issn.1000-1182.2012.06.020
局部应用血管内皮生长因子及骨髓间充质干细胞对超比例随意皮瓣成活率的影响
Topical application of vascular endothelial growth factor and bone marrow mesenchymal stem cell caused effects to the survival rate of random flap
摘要
Abstract
Objective Experimental study on the survival rate of random flap using vascular endothelial growth factor (VEGF) and bone marrow mesenchymal stem cell (MSC). Methods MSC was isolated through the combined utilization of density gradient centrifugation and adherence screening methods. MSC was cultured and its purity and biological properties were identified. Forty Wistar rat models were established and divided into control group, VEGF group, MSC group and MSC+VEGF group. Designed 2 cm×5 cm pedicle flap on each rat's back. The flaps were observed for 14 d after operation. On the 14th day, the rats were killed. The survival rates of random flap in each group were counted, and the histological sections were stained at the flap which was 4 cm far from the pedicle, and the number of microvascular was observed. Results 1)MSC was detected by flow cytometry, adipogenic and osteo-genic induction detection. The results showed that MSC could differentiate to fat and cartilage by induction and certified that the MSC had multi-differentiation potential. 2) Immune rejection did not appear on each animal. The survival rate of random flap in control group, VEGF group, MSC group and MSC+VEGF group were 55.4%±4.4%, 70.7%±6.3%, 65.1%±7.1%, and 93.4%±9.4%, respectively. The survival rate of each experimental group was higher than control group (P〈0.05). The survival rate of MSC+VEGF group was the highest (P〈0.05). 3)The histological examination showed that the granulation tissue, fibroblast and neovascularity was most abundant in MSC+VEGF group. Conclusion 1)The purified and multi-differentiated MSC can be obtained by density gradient centrifugation and ad-herence screening methods. 2) The human MSC has no immunogens or minimal immunogenicity, immunological rejection would not be caused when it is transmitted in cross-species. 3)VEGF and MSC have synergetic effect when they are used together, it can rebuild the blood circulation of the ischemic region in a short time, solve ischemia of the flap far from the pedicle, improve survival rate of the flap and enhance the repair quality of the wound.关键词
血管内皮生长因子/骨髓间充质干细胞/皮瓣Key words
vascular endothelial growth factor/ bone marrow mesenchymal stem cell/ skin flap分类
医药卫生引用本文复制引用
刘春丽,刘书会,刘志辉..局部应用血管内皮生长因子及骨髓间充质干细胞对超比例随意皮瓣成活率的影响[J].华西口腔医学杂志,2012,30(6):645-649,5.基金项目
吉林省科技厅白求恩医疗专项基金资助项目(200705345) （200705345）
