华西口腔医学杂志2013，Vol.31Issue(1)：8-12,5.DOI:10.7518/hxkq.2013.01.003
趋化因子受体CXCR4及其配体CXCL12在口腔鳞状细胞癌细胞增殖和迁移中的作用
The role of chemokine receptor CXCR4 and its ligand CXCL12 in the process of proliferation and migration of oral squamous cell carcinoma
摘要
Abstract
Objective To investigate the expression of chemokine receptor CXCR4 and its ligand CXCL12 in oral squamous cell carcinoma (OSCC) cells, and their effects on proliferation and migration of tumor cells. Methods The expression of CXCR4 mRNA and protein in 20 cases of OSCC tissue, cervical lymph nodes, tongue cancer cell line Tca8113, buccal cancer cell line Bca885, and 10 specimens of normal oral mucosa tissue were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot. The expression of CXCL12 mRNA was examined by RT-PCR. MTT assay was used to evaluate the CXCR4/CXCL12 influence on proliferation of tumor cells. Chemo-taxis and migration response to CXCR4 particular ligand-CXCL12 were detected by chemotaxis assay. Results DEx-pression levels of CXCR4 mRNA and protein in OSCC tissues, Tca8113 and Bca885 cells were 2.31±1.13, 1.89±1.20, 1.67±1.10 and 1.36±0.15, 1.85±0.34, 1.97±0.23, respectively. CXCR4 mRNA and protein couldn't be detected in normal tissues. CXCL12 mRNA level in cervical lymph nodes was 1.14±0.87, CXCL12 mRNA couldn't be detected in OSCC and normal tissues. 2) In MTT assay, recombinant CXCL12 stimulated proliferation of tumor cells and CXCR4 neutralization by monoclonal antibodies decreased proliferation. 3) The chemotactic migration of OSCC cells could be induced by CXCL12. CXCL12 at a concentration between 30 ng·mL-1 and 100 ng·mL-1 induced the chemotactic migration of OSCC cells in a dose-dependent manner. Conclusion CXCR4/CXCL12 system may promote proliferation and migration of tumor cells, and may play an important role in lymph node metastasis of OSCC.关键词
口腔鳞状细胞癌/趋化因子受体/增殖/迁移Key words
oral squamous cell carcinoma/chemokine receptor/proliferation/migration分类
医药卫生引用本文复制引用
尹东,张佐,高素,李斌..趋化因子受体CXCR4及其配体CXCL12在口腔鳞状细胞癌细胞增殖和迁移中的作用[J].华西口腔医学杂志,2013,31(1):8-12,5.基金项目
宁夏自然科学基金资助项目(NZ10181) （NZ10181）
