华西口腔医学杂志2013，Vol.31Issue(1)：91-95,5.DOI:10.7518/hxkq.2013.01.022
高迁移率族蛋白N2抑制口腔鳞状细胞癌增殖作用的体外研究
Study on prohibition of high mobility group chromosomal protein N2 against human oral squamous cell carcinoma in vitro
摘要
Abstract
Objective Take human oral squamous cell carcinoma Tca8113 as experimental model, and study the anti oral squamous cell carcinoma activity of high mobility group chromosomal protein N2(HMGN2) molecule. Methods Train a large number of recombinant human HMGN2 expression vector Escherichia coli BL21. HMGN2 was expressed under isopropyl-l-thio-p-galactopyranoside(IPTG) induction and purified by B-PER GST Fusion Protein Purification Kit A variety of concentrations HMGN2 were added to cell culture medium, cells were tested by MTT, Hoechst 33342 fluorescence staining, flow cytometry assay and Western-blot. Results MTT results proved that HMGN2 could significantly inhibit human oral squamous cell carcinoma Tca8113 growth. Hoechst 33342 fluorescence staining, flow cytometry assay test and Western-blot proved HMGN2 could make Tca8113 cells morphological change, make Tca8113 cells block in S period of cell cycle and strongly promote Tca8113 cells to apoptosis. Conclusion HMGN2 can promote apoptosis of oral squamous cell carcinoma cells.关键词
高迁移率族蛋白N2/人口腔鳞状细胞癌细胞株/凋亡Key words
high mobility group chromosomal protein N2/human oral squamous cell carcinoma cell line/apoptosis分类
医药卫生引用本文复制引用
董小倩,刘西茜,张永红,张平,卢礼兵,李小玉,黄萍,冯云..高迁移率族蛋白N2抑制口腔鳞状细胞癌增殖作用的体外研究[J].华西口腔医学杂志,2013,31(1):91-95,5.基金项目
国家自然科学基金资助项目(30972764) （30972764）
