四川大学学报（医学版）2013，Vol.44Issue(1)：21-26,6.
抗凋亡基因Bfl-1在前列腺癌中的表达及作用
The Expression and Function of Anti-apoptotic Bfl-1 in Prostate Cancer
摘要
Abstract
Objective To determine the expression of Bcl2 related protein Al (Bfl-l) mRNA in prostate cancer cell lines and tissues, and to explore the functions of Bfl-l in prostate adenocarcinoma. Methods RT-PCR, real-time quantitative PCR(Q-PCR) and in situ hybridization (ISH) were used to detect the expression of Bfl-l mRNA in prostate cancer cell lines, tissues and benign prostate hyperplasia (BPH) tissue samples. The relationship between Bfl-l expression and clinicopathological parameters was analyzed. Antisense oligonucleotides (ASONs) were used to interfere the expression of Bfl-l and its effects on prostate cancer cells. MTT was used to detect the survival, morphologic changes of prostate cancer cells was observed by inverted microscope. Results Bfl-l mRNA, detected by RT-PCR, Q-PCR and ISH, was overexpressed in the androgen-independent prostate cancer cell lines PC-3 and DU145, but not detectable in the androgen-dependent prostate cancer cell line LNCaP and BPH tissue samples (P<0. 05). Significantly higher Bfl-l mRNA levels were observed in higher stage and metastatic prostate cancer cases than those without metastasis or of low stage. ASONs targeting Bfl-l significantly inhibited androgen-independent prostate cancer cell growth (P<0. 05), cell was rounding off or fragmentation. Conclusion Bfl-l is involved in maintaining the hormone-independent prostate cancer cell growth. Bfl-l may become a new therapeutic target in advanced prostate cancer.关键词
Bcl2相关蛋白/A1/前列腺癌/细胞生长/反义寡核苷酸Key words
Bfl-l/Prostate cancer/Cell growth/Antisense oligonucleotides引用本文复制引用
李星斓,周桥,陈雪芹,聂玲,徐苗,李秋尧,商维维,陈铌,黄蕤,曾浩..抗凋亡基因Bfl-1在前列腺癌中的表达及作用[J].四川大学学报（医学版）,2013,44(1):21-26,6.基金项目
国家自然科学基金(No.30871383、30800637、31071134、81101529),高等学校博士学科点专项科研基金(No.20100181110019、20110181120021)和中国博士后科学基金(No.20100480076、201104643)资助 （No.30871383、30800637、31071134、81101529）
