海洋科学2012，Vol.36Issue(8)：35-39,5.
海洋放线菌不同分离方法的比较研究
Comparative study of different methods for isolation of marine actinomycetes
摘要
Abstract
The isolation techniques for marine actinomycetes from the inter-tidal sediment at Qingdao were studied by using the series dilution and plate spreading methods. The impacts of different pretreatments, diluents, seawater concentrations and media on the isolation of actinomycetes were investigated. The results showed that the growth of bacteria were obviously restrained in the samples when pretreated with 55℃ for 6 minutes, which enhanced the isolation of actinomycetes from the inter-tidal sediment; dilution of the samples with 1/4 Ringer's solution and spreading them on the media prepared with pure seawater could improve the isolation of actinomycetes. The 9 media exhibited significant differences on the number of actinomycetes recovered, with media Ml, M6, M7 and M8 being more effective than others.关键词
海洋沉积物/放线菌/分离方法Key words
marine sediment/ actinomycete/ isolation method分类
海洋科学引用本文复制引用
常显波,刘文正,尹琦,张晓华..海洋放线菌不同分离方法的比较研究[J].海洋科学,2012,36(8):35-39,5.基金项目
国家高技术研究发展计划资助项目(2007AA09Z434) （2007AA09Z434）
国家海洋局海洋生物活性物质与现代分析技术重点实验室开放基金资助项目(MBSMAT-2010-02) （MBSMAT-2010-02）
烟台大学博士启动基金资助项目(HJ11B26) （HJ11B26）
