基础医学与临床2013，Vol.33Issue(1)：44-48,5.
油酸调节人血管平滑肌细胞TLR4的增殖活性及炎性因子的表达
Oleic acid increases the expression of TLR4 and inflammatory cytokines mRNA and proteins in HA-VSMC
摘要
Abstract
Objective To investigate the regulation of TLR4 and inflammatory cytokines by oleic acid in human aortic vascular smooth muscle cells ( HA-VSMCs). Methods MMT method was used to analyze the proliferation ability of human aortic vascular smooth muscle cells. TLR4, IL-6, IL-8 and MCP-1 mRNA expressions were detected by quantitative real-time PCR. TLR4, IL-6, IL-8 and MCP-1 protein was measured by Western blot and ELISA. Results Oleic acid induced the proliferation of HA-VSMC, but the proliferation ability of cells was inhibited with rising of dose age. Oleic acid upregulated the expression of TLR4, IL-6, IL-8 and MCP-1 mRNA by 6. 5 fold,7. 4 fold,7. 4 fold and 7. 1 fold, and the levels of IL-6, IL-8 and MCP-1 proteins were significantly enhanced by 2. 16 fold, 1. 93 fold and 2. 06 fold (all P 〈 0. 05 ). The levels of TLR4 protein (1. 70 ± 0. 62) were markedly augmented by 200 μmol/L oleic acid than that of control (0. 29 ± 0. 22) ( P 〈 0. 05). LPS markedly increased TLR4, IL-6, IL-8 and MCP-1 expression in HA-VSMC (P 〈 0. 05). Conclusions Oleic acid induces the expression of TLR4 and in flammatory cytokines mRNA and proteins in HA-VSMC.关键词
人主动脉血管平滑肌细胞/油酸/果蝇样受体4/细胞因子/基因表达Key words
human aortic vascular smooth muscle cells /oleic acid/Toll like receptor 4/cytokine/gene expression分类
医药卫生引用本文复制引用
杨海静,陈卫,权金星,李伟华,刘静,邱明才..油酸调节人血管平滑肌细胞TLR4的增殖活性及炎性因子的表达[J].基础医学与临床,2013,33(1):44-48,5.基金项目
国家自然科学基金(30960147) （30960147）
甘肃省自然科学基金(1010RJZA178) （1010RJZA178）
