基础医学与临床2013，Vol.33Issue(1)：70-76,7.
4.1蛋白家族成员merlin与ezrin在青海藏族胃癌中表达及对胃癌细胞功能的影响
The expressions of 4.1 protein family member merlin and ezrin in gastric cancer of qinchai tibetan patients and its influences on the cellular functions of gastric cancer cell
摘要
Abstract
Objective To study the mutation of tumor suppressor protein merlin and the expression of ezrin in gastric cancer of Qinghai Tibetan patients, meanwhile to explore the influences of merlin and ezrin on the proliferation and adhesion ability of gastric cancer cell. Methods PCR and DNA sequencing were used to analyze the mutation, and the ezrin expression was detected by immunochemical staining. The proliferation and adhesion ability of gastric cancer cell were analyzed by MTT assay. Results The mutation had been detected in merlin gene NF2 exons, and two sites in exon 17 showed high mutation rate, it was more than 50%. Ezrin protein expression was higher in gastric cancer than normal gastric mucosa in Qinghai Tibetan patients (P 〈0. 05). The expression level of ezrin protein was correlated with tumor differentiation, lymph node metastasis and histological type of Qinghai Tibetan patients (P 〈0. 05). Furthermore, MTT assay showed that FERM domain and merlin-2 had no significant influences on the proliferation and adhesion ability of gastric cancer cell, and ezrin showed no effect on the adhesion a-bility of gastric cancer cell, but it showed ezrin influenced the cell proliferation. Tumor suppressor protein merlin-1 showed not only a much stronger inhibition of proliferation, but also decreased the cell adhesion ability of gastric cancer cell, and the inhibition of proliferation by merlin was much stronger than ezrin protein. Conclusions The expression of merlin and ezrin is related with the susceptibility to gastric cancer development and metastasis in Qinghai Tibetan patients. Furthermore, merlin-1 and ezrin are involved in adjustment of proliferation and adhesion ability of gastric cancer cell.关键词
merlin/ezrin/青海藏族/胃癌/易感性Key words
merlin/ ezrin/ Qinghai Tibetan/ gastric cancer/ susceptibility分类
医药卫生引用本文复制引用
苏占海,杨生玺,王荣华,顾烨,马红英,孔繁花..4.1蛋白家族成员merlin与ezrin在青海藏族胃癌中表达及对胃癌细胞功能的影响[J].基础医学与临床,2013,33(1):70-76,7.基金项目
国家自然基金项目(81160259) （81160259）
青海省科技厅项目(2010-Z-721) （2010-Z-721）
