基础医学与临床2013，Vol.33Issue(1)：82-87,6.
PKC/NADPH氧化应激途径对大鼠心脏微血管内皮细胞eNOS脱偶联的影响
Effects of PKC/NADPH oxidase on AGEs-induced eNOS uncoupling in rat cardiac microvascular endothelial cells
摘要
Abstract
Objective To observe the effects of PKC/NADPH oxidase on AGEs-induced eNOS uncoupling in rat cardiac microvascular endothelial cells. Methods To culture the cardiac microvascular endothelial cells in vitro. AGEs(100 mg/L) , DPI and LY33531 (2. 5,5 and 10 μmol/L)was respectively added and resumed incubation for 24 hrs. BH4 , NO, O-2 generation, eNOS, ROS and P47phox protein expression were examined. Results As LY33531 and DPI concentration was increased, production of NO was noted increased gradually (90. 7 ± 0. 3 ~ 122. 6 ± 0. 3,160. 6 ± 0. 6, P 〈 0. 05) , while production of 0-2 was decreased gradually (P 〈 0. 05) , BH4 expres-sion was increased(P 〈0. 05) , eNOS expression was gradually decreased ( 126. 1 ±3.5 ~112. 6 ±1.7,114. 4 ± 1.7,P〈0. 05). Meanwhile, P47phox protein expression and ROS decreased gradually (P〈0. 05). Conclusions AGEs may trigger the activation of PKC/NADPH oxidative stress pathway inducing intracellular eNOS uncoupling in cardiac microvascular endothelial cells.关键词
糖基化终末产物/心脏微血管内皮细胞/一氧化氮/内皮型一氧化氮合酶/PKC/NADPH/LY33531/DPIKey words
BSA-AGEs/ cardiac microvascular endothelial cells/ NO/ eNOS/ PKC/NADPH/LY33531/DPI分类
医药卫生引用本文复制引用
成永霞,刘贵波,颜彬,冯玉宽,郭素芬,王宏伟,杨向红..PKC/NADPH氧化应激途径对大鼠心脏微血管内皮细胞eNOS脱偶联的影响[J].基础医学与临床,2013,33(1):82-87,6.基金项目
教育部科学技术研究重点项目(211050) （211050）
黑龙江省青年科学基金(QC2010040) （QC2010040）
