基础医学与临床2013，Vol.33Issue(2)：133-138,6.
Müller细胞反应性胶质化在急性高眼压致大鼠视网膜损伤中作用
Role of Müller cell-specific reactive gliosis in acute ocular hypertension-induced retinal damage of rats
摘要
Abstract
Objective To observe Müller cell-specific reactive gliosis in rat retina following acute ocular hypertension ( AOH) and its effect on AOH-induced retinal damage. Methods The AOH rat model was established, and the animals were divided into control (Ctrl) , AOH and AOH + α-aminoadipic acid (AAA, intravitreal injection) treated groups (1,3 and 5d subgroups according the reperfusion time) , AAA treatment or AOH + PBS control group. TUNEL assay was used to detect cell apoptosis, GFAP immunofluorescent staining was performed to respond Miiller cell-specific reactive gliosis, and Thy-1 staining was applied to mark retinal ganglion cells (RGCs). Results AOH attenuated the thicknesses of inner plexiform layer (IPL) and inner nuclear layer (INL) , and cause cell disorganization and cell loss in ganglion cell layer (GCL) as well as the Müller cell-specific reactive gliosis ( enhanced GFAP-immunoreactivity) in rat retina. In addition, the inhibition of Müller cell-specific reactive gliosis by AAA intravitreal injection did significantly relief RGCs loss and cell apoptosis in GCL layer of AOH rat retina. Conclusions Müller cell-specific reactive gliosis is involved in AOH-induced retinal damage and its inhibition may be a potential therapeutic strategy for improving glaucomatous retinopathy.关键词
急性高眼压/视网膜/Müller细胞/视网膜神经节细胞Key words
acute ocular hypertension/ retina/ Müller cells/ retinal ganglion cells分类
医药卫生引用本文复制引用
苏吉儿,丁静文,韩松,罗宏,李俊发..Müller细胞反应性胶质化在急性高眼压致大鼠视网膜损伤中作用[J].基础医学与临床,2013,33(2):133-138,6.基金项目
国家自然科学基金(81050003,30871219,31071048,31171147) （81050003,30871219,31071048,31171147）
教育部高等学校博士点科研基金(20091107110001) （20091107110001）
北京市属高等学校人才强教深化计划创新人才(PHR200906116) （PHR200906116）
