基础医学与临床2013，Vol.33Issue(2)：144-148,5.
H2-Calponin蛋白在4种癌组织的表达及临床意义
Expression of H2-Calponin protein in four cancerous tissues and its clinical significance
摘要
Abstract
Objective To explore the expression and distribution of H2-Calponin mRNA and protein in four cancerous tissues and its clinical significance. Methods In situ RT-PCR was used to detect the expression and distribution of H2-Calponin mRNA in four different cancerous tissues. Immunohistochemistry was used to detect the expression of H2-Calponin protein in lung cancer, gastric carcinoma, nasopharyngeal carcinoma(NPC) and hepatocellu-lar carcinoma( HCC). Results H2-Calponin protein was mainly expressed in cytoplasm by immunohistochemistry. The positive rates of H2-calponin protein in lung cancer, gastric carcinoma, nasopharyngeal carcinoma and HCC were 30. 0% ,27. 5% ,45. 0% and 51. 8% ,respectively. The expression positive rate of H2-Calponin in HCC was the highest. The expression of H2-Calponin mRNA was showed near the nucleus by in situ RT-PCR. The mRNA expression rates of H2-Calponin in lung cancer, gastric carcinoma,nasopharyngeal carcinoma and HCC were 17. 5% , 10. 0% ,20. 0% and 50. 0% ,respectively. The positive rate was the highest H2-Calponin in HCC. The positive rate of H2-calponin protein in HCC with metastasis was higher than that without metastasis. The expression of H2-Cal-ponin protein in four different cancerous tissues was not statistically different with patient gender,age and pathologic differentiation grade. Conclusions H2-Calponin protein is correlated with HCG, specially positively correlated with HCC with metastasis.关键词
H2-Calponin/肝癌/免疫组织化学/原位RT-PCRKey words
H2-Calponin/ hepatocarcinoma/ immunohistochemistry/ in situ RT-PCR分类
医药卫生引用本文复制引用
李玲,涂镇波,张永聪,陶欢,单安祺,黎业源,周素芳..H2-Calponin蛋白在4种癌组织的表达及临床意义[J].基础医学与临床,2013,33(2):144-148,5.基金项目
国家自然科学基金(81160362) （81160362）
广西自然科学基金(2011GXNSFA018261) （2011GXNSFA018261）
广西科学研究与技术开发计划(桂科攻10124001A-2) （桂科攻10124001A-2）
