基础医学与临床2013，Vol.33Issue(2)：199-204,6.
JNK信号通路介导高糖诱导的大鼠心肌成纤维细胞增殖
C-JUN N-terminal kinase pathway mediates high glucose-induced proliferation of cardiac fibroblasts in rats
摘要
Abstract
Objective To determine whether JNK signaling pathway is involved in high glucose (HG) -induced proliferation of cardiac fibroblasts (CFs) in rats. Methods Rat CFs were cultured in DMEM (NG: 5.5 mmol/L D-glucose; HG: 12, 18, 25 mmol/L D-glucose; OSM: 5.5 mmol/L D-glucose + 19.5 mmol/L mannitol) for indicated time periods(0, 12, 24, 48 h). Meanwhile, rat CFs were cultured in DMEM (25 mmol/L D-glucose) with SP600125(10, 20 μmol/L), a JNK inhibitor, for 48 h. Proliferation was measured by MTT. The expression of proliferating cell nuclear antigen (PCNA), total JNK and phosphorylation of JNK were detected by Western blot. C-JUN mRNA expression was assessed by RT-PCR. Results Treatment of CFs with HG significantly increased phosphorylation of JNK in time-and glucose concentration-dependent manner. Compared with NG group, exposure of CFs to HG(25 mmol/L) significantly promoted the proliferation of CFs(0.44 ± 0. 02 vs 0. 31 ± 0. 02, P 〈 0.01), and upregulated C-JUN mRNA expression and PCNA levels. SP600125 significantly suppressed HG-induced the proliferation of CFs and the activity of JNK in a dose-dependent manner. Conclusion HG stimulates the proliferation of CFs partially through JNK pathway activation.关键词
葡萄糖/心肌成纤维细胞/增殖/C-JUN氨基末端激酶Key words
glucose/ cardiac fibroblasts/ proliferation/ C-JUN N-terminal kinase pathway分类
医药卫生引用本文复制引用
刘丽华,房彩霞,邓永贵,周红..JNK信号通路介导高糖诱导的大鼠心肌成纤维细胞增殖[J].基础医学与临床,2013,33(2):199-204,6.基金项目
河北省卫生厅重大课题资助项目(20090007) （20090007）
