解放军医学杂志2013，Vol.38Issue(1)：15-18,4.
香椿子正丁醇提取物对脑缺血再灌注小鼠的神经保护作用及其机制研究
Neuroprotective effect of n-butanol extract of Chinese toon seeds on mouse with injury of ischemia-reperfusion and its mechanism
摘要
Abstract
Objective To investigate the neuroprotective effect and its mechanism of n-butanol extract (n-BuE) of Chinese toon seeds on mouse with ischemia-reperfusion (I-R) injury of the brain in vivo. Methods The brain I-R model was established in 42 mice by ligation of bilateral common carotid arteries for 2 times combined with injection of sodium nitroprusside. Animals were randomly divided into 6 groups (7 each): sham-operated group, I-R model group, vehicle control group, two treatment groups [treated with n-BuE of Chinese toon seeds 28mg/(kg·d) or 42mg/(kg·d), respectirely, for 7 days], and positive control group [treated with aspirin 14mg/(kg·d)]. The animals were sacrificed by decapitation after the experimental procedure, and the water content of brain tissue was determined by weighing. The permeability of the blood brain barrier was evaluated by measurement of Evans blue (EB) content with spectrophotometer. The contents of malondialdehyde (MDA) and nitrogen mono-oxide (NO), and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in brain tissue were determined with spectrophotometer. Results The water content and EB content of brain tissue, and the contents of MDA and NO, and the activities of SOD and GPX in hippocampal tissue increased significantly in I-R model group than in sham-operation group (P<0.05 or P<0.0l), while they decreased obviously in the two treatment groups when compared with those in I-R model group (P<0.05 or P<0.0l). Conclusion n-BuE of Chinese toon seeds may play a neuroprotective effect on the brain tissue I-R injured mice due to its anti-oxidative stress effect.关键词
香椿子/脑缺血/再灌注损伤Key words
Chinese toon seeds/ brain ischemia/ reperfusion injury分类
医药卫生引用本文复制引用
马迪,陈超,吴薇,游艳,潘宜云,盛梦婷,朱峰,何治..香椿子正丁醇提取物对脑缺血再灌注小鼠的神经保护作用及其机制研究[J].解放军医学杂志,2013,38(1):15-18,4.基金项目
国家自然科学基金青年基金(81100873) （81100873）
湖北省卫生厅青年科技人才项目(QJX2010-27) （QJX2010-27）
三峡大学博士科研启动基金(KJ2008B053) （KJ2008B053）
