激光生物学报2012，Vol.21Issue(5)：425-428,4.DOI:10.3969/j.issn.1007-7146.2012.05.008
不同营养水平下绵羊附睾头GPx5表达与定位
Expression and Locational Analysis of GPx5 in the Caput Epididymidis of Sheep Feeding Diet with Different Nutrition Levels
摘要
Abstract
Total RNA was isolated from caput epididymidis of sheep feeding diet with different nutrition levels and mR-NA expression was detected by real-time fluorescent quantitative PCR. Localization of GPx5 in caput epididymidis was examined respectively by immunohistochemisty. The result showed that the quantitative real-time PCR results showed that the rank order of GPx5 mRNA expression was 35%VFI group < 65% VFI group < Voluntary feed intake( VFI ). The immunofluorescence displayed positive expressions of GPx5 in epithelial cells of caput epididymidis. GPx5 appeared to be differently expressed at different nutrition levels and low nutrition level significantly influenced expression of GPx5 in caput epididymidis.关键词
营养/GPx5/附睾头/绵羊Key words
nutrition/ GPx5 / caput epididymidis/ sheep分类
农业科技引用本文复制引用
程彩虹,张春香,任有蛇,岳文斌..不同营养水平下绵羊附睾头GPx5表达与定位[J].激光生物学报,2012,21(5):425-428,4.基金项目
现代农业产业技术体系建设专项资金(NYCYTX-39) （NYCYTX-39）
