激光生物学报2012，Vol.21Issue(5)：446-452,457,8.DOI:10.3969/j.issn.1007-7146.2012.05.012
毕赤酵母发酵产S-腺苷蛋氨酸的甘油-甲醇混合流加策略
Glycerol-methanol Mixed-feeding Strategy for S-adenosyl-L-methionine Fermentative Production by Pichia pastoris
摘要
Abstract
Mixed carbon sources culture medium with different ratio of glycerol to methanol was evaluated in the S-ade-nosyl-L-methionine ( SAM ) fermentative production by recombinant Pichia pastoris. When the supplement mixed medium contained 10% ( w/v ) glycerol, SAM production reached 6. 09 g/L, which was 20. 4% higher than the production of feeding 0% glycerol contained medium. On that basis, two sets of glycerol-methanol mixed-feeding strategy were performed at the 7 L fermentor to improve SAM production. When 100% methanol was fed into bioreactor for 24 h before 10% ( w/v ) glycerol content of mixed medium was put into use, SAM production was raised to 7. 94 g/L. When different glycerol content of mixed medium was fed into bioreactor at five different sections during the methanol induction phase, SAM production reached the highest 9. 80 g/L.关键词
毕赤酵母/S-腺苷蛋氨酸/发酵/混合流加策略Key words
Pichia pastoris/ S-adenosyl-L-methionine/ fermentation/ mixed-feeding strategy分类
生物科学引用本文复制引用
肖安风,许海琴,倪辉,杜希萍,蔡慧农..毕赤酵母发酵产S-腺苷蛋氨酸的甘油-甲醇混合流加策略[J].激光生物学报,2012,21(5):446-452,457,8.基金项目
福建省自然科学基金项目(2011J01224) （2011J01224）
集美大学中青年创新团队专项基金项目(No.2010A006) （No.2010A006）
集美大学科研基金项目(ZQ2006043) （ZQ2006043）
