吉林大学学报（理学版）2013，Vol.51Issue(1)：140-144,5.
树舌荧光多糖的制备及其在人大肠癌细胞SWWC1116中的定位
Preparation of Fluorescent Polysaccharides from Ganoderma applanatum and Cellular Localization in Human Colorectal Cancer SWWC1116
摘要
Abstract
The reductive amination method was used for the fluorescently labelling of Ganoderma applanatum polysaccharide(GAP). FITC was linked up with the reducibility termination of GAP, and fluorescent substitute ratio was determined to be 0. 90%. The results of the measurement by xCELLigence RTCA DP automatic real-time cell analyzer show in vitro the cytotoxic activity did not significantly change before and after fluorescent labeling of GAP. The results measured by means of flow cytometer and fluorescence microscope show fluorescence signal was detected both-on the cell membrane and in the nucleus, illustrating that GAP can be attached to human colorectal cancer SWWC1116 membrane, and can be further transported to the nuclei.关键词
树舌/多糖/荧光标记/大肠癌细胞SWWC1116/细胞定位Key words
Ganoderma applanatum/ polysaccharide/ fluorescent labelling/ human colorectal cancer SWWC1116/ cellular localization分类
生物科学引用本文复制引用
苏玲,李雨婷,王再林,宋慧,杜金,吴宗翰,张中北..树舌荧光多糖的制备及其在人大肠癌细胞SWWC1116中的定位[J].吉林大学学报（理学版）,2013,51(1):140-144,5.基金项目
吉林省世行贷款农产品质量安全项目(批准号:2011-Y18). （批准号:2011-Y18）
