吉林农业大学学报2012，Vol.34Issue(6)：661-666,6.
猪繁殖与呼吸综合征病毒核蛋白单克隆抗体的制备和初步鉴定
Preparation and Identification of the Monoclonal Antibodies against the Nucleoprotein of Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
The full sequence of the nucleoprotein gene of porcine reproductive and respiratory syndrome virus strain CC-11 was cloned and inserted into the expression vector pET28a. The resultant expression plasmid was transformed into E. coli Rosetta and induced by 0.5 mmol/L IPTG. High level expression of the nucleoprotein was achieved. After purification of the nucleoprotein by Ni affinity chromatography column , it was used to vaccinate BALB/c mice by 50 μg for 3 times. The spleen cells were fused with SP2/ 0 cells and after rounds of screening by indirect ELISA, 3 hybridoma cell lines, named as 9B12, 7C2, 7C6, secreting monoclonal antibodies against PRRSV nucleoprotein, were obtained. The subtypes of the monoclonal antibodies are IgG2b, IgG2a and IgGl, respectively. These monoclonal antibodies can react with the PRRSV stains CC-11, CH-1R, and JXA1-R maintained in our laboratory and the 4 suspected clinical specimens.关键词
猪繁殖与呼吸综合征病毒/N蛋白/单克隆抗体Key words
porcine reproductive and respiratory syndrome virus/ nucleoprotein/ monoclonal antibody分类
农业科技引用本文复制引用
米立娟,吕宗吉,张守峰,刘晔,王永志,扈荣良..猪繁殖与呼吸综合征病毒核蛋白单克隆抗体的制备和初步鉴定[J].吉林农业大学学报,2012,34(6):661-666,6.基金项目
农业部公益性行业项目(201203056) （201203056）
