吉林农业大学学报2012，Vol.34Issue(6)：677-681,5.
脑源性神经营养因子在猪卵泡中的表达
Expression of Brain Derived Neurophic Factor (BDNF) in Porcine Follicles
摘要
Abstract
In order to determine the effect of BDNF on reproductive system, expression of BDNF was detected in different sized swine follicle at follicular phase and luteal phase. RT-PCR, ELISA were used to examine the expression of BDNF in the follicular granulosa cells and follicular fluid of pocine. The result shows that BDNF was expressed in every stage of follicular granulosa cells and follicular fluid. The level of BDNF mRNA was significantly different in follicles among different sizes, in which the highest level was found in large follicles. The level of BDNF protein was at high level in follicle of every stage. The concentration of BDNF in medium follicle in luteal phase was higher than that in follicular phase, which is different from that of granulosa cells.关键词
脑源性神经营养因子/猪/颗粒细胞Key words
BDNF/ swine/ granulosa cell分类
农业科技引用本文复制引用
芭帕婉,侯晓峰,王春强,李万宏,陈超,周虚..脑源性神经营养因子在猪卵泡中的表达[J].吉林农业大学学报,2012,34(6):677-681,5.基金项目
国家重点基础研究发展计划项目(2011CB944203) （2011CB944203）
