吉林农业大学学报2013，Vol.35Issue(1)：15-18,4.
利用绿色荧光蛋白(GFP)标记香蕉枯萎病菌及其稳定性检测
Marking of Fusarium oxysporum f.sp.cubense with Green Fluorescent Protein (GFP) and Measuement of Its Stability
摘要
Abstract
Plasmid pSCOOl carrying GFP gene transformation was transferred into Fusarium oxysporum f. sp. cubense(Foc)using PEG-CaCl2. Observation under fluorescent microscope showed that when transfor-mants were excitated by blue light, hypha and spores all porduced stable green fluorescent, indicating that GFP gene was successfully transformed to Foe and expressed. Pathogens marked with GFP were continuously cultured in later generations and vaccinated to Brazilian banana. The results showed that the introduction of GFP did not significantly affect the pathogenicity and pathogenicity of pathogens.关键词
香蕉枯萎病/绿色荧光蛋白GFP/基因转化/原生质体Key words
Fusarium wilt of banana (Panama disease) / green fluorescent protein/ gene transformation/protoplast分类
农业科技引用本文复制引用
刘勇勤,李赤,徐秀德,刘菲菲,于莉..利用绿色荧光蛋白(GFP)标记香蕉枯萎病菌及其稳定性检测[J].吉林农业大学学报,2013,35(1):15-18,4.基金项目
湛江市科技局项目(2008) （2008）
